Plasmid

Part:BBa_I751001:Design

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-23)


pBR Biobrick-Tokyo Standard


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2762
    Illegal SpeI site found at 2831
    Illegal PstI site found at 2845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2762
    Illegal SpeI site found at 2831
    Illegal PstI site found at 2845
    Illegal NotI site found at 2768
    Illegal NotI site found at 2838
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2762
    Illegal BglII site found at 2810
    Illegal BamHI site found at 2798
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 2831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2762
    Illegal XbaI site found at 2777
    Illegal SpeI site found at 2831
    Illegal PstI site found at 2845
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2003
    Illegal SapI site found at 920


Design Notes

We removed 1618bp from pBR322 (from SspI to AvaI site). So this plasmid lost Tet resistant gene.

This plasmid have Amp resistant gene only.


Source

pBR322 dPst (BBa_I751000)

References